پاورپوینت Photosynthesi The Hill Reaction
پاورپوینت Photosynthesi The Hill Reaction
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پاورپوینت Photosynthesi The Hill Reaction (pptx) 24 اسلاید
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قسمتی از متن PowerPoint (.pptx) : Photosynthesis II: The Hill ReactionWhat’s the point of this lab?Understand the electron transport chainAlter variables in the ETC to observe outcomeDevelop methods to determine order of events in the ETCReminders6CO2 + 12H2O + light C6H12O6 + 6O2 + 6H2OExcitation Chlorophyll (water split) ETC (PQ as H+ pump) Excitation Chlorophyll ETC Reduction NADP+ to NADPH + H+ (stroma)High H+ concentration in thylakoid interior causes ATP production (stroma)Electron Transport ChainDCPIP – the electron theifHigh absorbance at 600 nmBlue = Oxidized (electron poor)Clear = Reduced (electron rich)Hill Reaction (Figure 7.1)Review of Hill ReactionIsolated chloroplasts could be stimulated by light to transfer e- through ETC to an artificial electron acceptorWe measure the Hill Reaction with other e- acceptorsHow do herbicides affect the ETC?DCPIP: artificial e- acceptor from ETCHerbicide: blocks ETCHill Reactionhttp://www.life.uiuc.edu/govindjee/photoweb/NewZScheme.jpgDCPIP(Hill Reaction)DCPIPBlueOxidized (no e-)ColorlessReduced (gains e-)If Photosystem II is not stopped and DCPIP is allowed to steal electrons and unclog the e- back-up in the ETC then H2O is split into 2 H+ and O2 and e-'s are moved down the chain.Electron Transport Chain
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